Journal: Scientific Reports

Article Title: Autoimmune reactions to ACE2 triggered by SARS-CoV-2 S protein and their histopathological consequences

doi: 10.1038/s41598-025-21304-y

Figure Lengend Snippet: Time course of antibodies to SARS-CoV-2 S protein and autoantibodies to ACE2 in rats immunized with SARS-CoV-2 S protein. The data are presented as mean ± SD. n is the number of rats in the group.

Article Snippet: Type 2 pneumocytes were stained with rabbit polyclonal antibodies to ACE2 (Affinity Biosciences Ltd., Cincinnati, USA) or rabbit polyclonal antibodies to SP-C (Affinity Biosciences Ltd., Cincinnati, USA).

Techniques:

Journal: Scientific Reports

Article Title: Autoimmune reactions to ACE2 triggered by SARS-CoV-2 S protein and their histopathological consequences

doi: 10.1038/s41598-025-21304-y

Figure Lengend Snippet: Lung sections of rats immunized with SARS-CoV-2 S protein. ( a ) ACE2 positive cells in the focus of alveolar hyperplasia in SARS-CoV-2 S protein immunized rat. b. ACE2 positive cells in normal lung of IFA injected rat. c. Focus of hyperplasia in SARS-CoV-2 S protein immunized rat. Arrowhead—mitotic figure. Open arrow—binucleated cell. ( a , b ) Lung sections were treated with rabbit antibodies to ACE2 and HRP-conjugated goat anti-rabbit IgG, IgM, IgA antibodies. Arrows—ACE2 expressing cells (type II pneumocytes). ( c ) H&E staining.

Article Snippet: Type 2 pneumocytes were stained with rabbit polyclonal antibodies to ACE2 (Affinity Biosciences Ltd., Cincinnati, USA) or rabbit polyclonal antibodies to SP-C (Affinity Biosciences Ltd., Cincinnati, USA).

Techniques: Injection, Expressing, Staining